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Galectin Therapeutics recombinant galectin 1
Recombinant Galectin 1, supplied by Galectin Therapeutics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+galectin-1/lgals3/bio_rxiv__64898__2026__02__23__707584-417-7-8
Average 86 stars, based on 1 article reviews
recombinant galectin 1 - by Bioz Stars, 2026-10
86/100 stars

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Article Title: S100A8, S100A9 and S100A8/A9 heterodimer as novel cachexigenic factors for pancreatic cancer-induced cachexia.
Article Snippet: Preparation of recombinant candidate proteins Recombinant His-tagged S100A9 and galectin-1 were prepared as described previously [16].

Article Title: Endogenous Galectin-1 Modulates Cell Biological Properties of Immortalized Retinal Pigment Epithelial Cells In Vitro.
Article Snippet: In contrast, following incubation with 0.5 μg/mL recombinant galectin-1, the number of attached galectin-1deficient cells decreased by 17% when compared to untreated ARPE-19-LGALS1−/− cells (45 ± 3%; p < 0.001; Figure 5E).

Article Title: Endogenous Galectin-1 Modulates Cell Biological Properties of Immortalized Retinal Pigment Epithelial Cells In Vitro
Article Snippet: In contrast, following incubation with 0.5 μg/mL recombinant galectin-1, the number of attached galectin-1-deficient cells decreased by 17% when compared to untreated ARPE-19-LGALS1 −/− cells (45 ± 3%; p < 0.001; E).

Article Title: Mucins form a nanoscale material barrier against immune cell attack
Article Snippet: Treatment of wild-type 1E7 cells 219 with recombinant galectin-3, recombinant galectin-1, or 10 m of the small-molecule galectin 220 inhibitor TD139 (Kd = 0.036 M for galectin-3; Kd = 2.2 M for galectin-1) did not significantly 221 change the material thickness of the glycocalyx at 1,000 ng/mL doxycycline induction (Fig. 2E).

Article Title: N-glycosylation engineering in chimeric antigen receptor T cells enhances anti-tumor activity
Article Snippet: As positive control, cells were incubated with recombinant Galectin-1 or -3 before performing the cell surface staining.

Recombinant:

Article Title: Probing the Ca V 1.2 interactome in heart failure identifies a positive modulator of inotropy and lusitropy
Article Snippet: After blocking with 3% bovine serum albumin (MP Biomedicals, 9048-46-8), cells were incubated overnight with streptavidin Alexa FluorTM 488 (Thermo Fisher, S11223) at 1.3μg/mL and imaged with confocal microscopy. .. Because Gal-1 is reported to dimerize through its C-terminus, and recombinant Gal-1 has C-terminal fusion of a linker and 3XFLAG motif, we modeled recombinant Galectin-1 dimerization with AlphaFold Multimer. ..

Article Title: Protein purification strategies must consider downstream applications and individual biological characteristics
Article Snippet: A different problem arose with the prolonged preparation of the protein—in the absence of any reducing agent, the wild-type Galectin-1 construct completely oxidized following purification, and even in its presence, the protein remained reduced for a short time only (Fig. ). .. Knowing that the preparation of recombinant Galectin-1 was in our particular case intended for ligand binding studies with proteins containing disulfide bonds, the presence of reducing agents, even at a lower concentration, could not be considered to avoid destabilizing the binding partner. .. It became evident that the best candidate for a folded, functional, and stable protein was the cysteine-less mutant of Galectin-1 purified by affinity chromatography over a lactose-based column and dialyzed for removal of lactose.

Article Title: Lactose blocks intercellular spreading of Galectin-1 from cancer cells to T-cells and activates tumor immunological control
Article Snippet: .. Surprisingly, FACS staining with biotin-labeled recombinant Galectin-1 showed a more dramatic difference between control and B4GALT1 knockout OT-1 T-cells ( and Fig. S4c). .. RNA sequencing analysis revealed that galectin-1, galectin-3, and galectin-9 (Gal-1, Gal-3, Gal-9) were the most highly expressed galectins in OT-1 T-cells and MC38 cells (Fig. S5a).

Ligand Binding Assay:

Article Title: Protein purification strategies must consider downstream applications and individual biological characteristics
Article Snippet: A different problem arose with the prolonged preparation of the protein—in the absence of any reducing agent, the wild-type Galectin-1 construct completely oxidized following purification, and even in its presence, the protein remained reduced for a short time only (Fig. ). .. Knowing that the preparation of recombinant Galectin-1 was in our particular case intended for ligand binding studies with proteins containing disulfide bonds, the presence of reducing agents, even at a lower concentration, could not be considered to avoid destabilizing the binding partner. .. It became evident that the best candidate for a folded, functional, and stable protein was the cysteine-less mutant of Galectin-1 purified by affinity chromatography over a lactose-based column and dialyzed for removal of lactose.

Concentration Assay:

Article Title: Protein purification strategies must consider downstream applications and individual biological characteristics
Article Snippet: A different problem arose with the prolonged preparation of the protein—in the absence of any reducing agent, the wild-type Galectin-1 construct completely oxidized following purification, and even in its presence, the protein remained reduced for a short time only (Fig. ). .. Knowing that the preparation of recombinant Galectin-1 was in our particular case intended for ligand binding studies with proteins containing disulfide bonds, the presence of reducing agents, even at a lower concentration, could not be considered to avoid destabilizing the binding partner. .. It became evident that the best candidate for a folded, functional, and stable protein was the cysteine-less mutant of Galectin-1 purified by affinity chromatography over a lactose-based column and dialyzed for removal of lactose.

Binding Assay:

Article Title: Protein purification strategies must consider downstream applications and individual biological characteristics
Article Snippet: A different problem arose with the prolonged preparation of the protein—in the absence of any reducing agent, the wild-type Galectin-1 construct completely oxidized following purification, and even in its presence, the protein remained reduced for a short time only (Fig. ). .. Knowing that the preparation of recombinant Galectin-1 was in our particular case intended for ligand binding studies with proteins containing disulfide bonds, the presence of reducing agents, even at a lower concentration, could not be considered to avoid destabilizing the binding partner. .. It became evident that the best candidate for a folded, functional, and stable protein was the cysteine-less mutant of Galectin-1 purified by affinity chromatography over a lactose-based column and dialyzed for removal of lactose.

FACS:

Article Title: Lactose blocks intercellular spreading of Galectin-1 from cancer cells to T-cells and activates tumor immunological control
Article Snippet: .. Surprisingly, FACS staining with biotin-labeled recombinant Galectin-1 showed a more dramatic difference between control and B4GALT1 knockout OT-1 T-cells ( and Fig. S4c). .. RNA sequencing analysis revealed that galectin-1, galectin-3, and galectin-9 (Gal-1, Gal-3, Gal-9) were the most highly expressed galectins in OT-1 T-cells and MC38 cells (Fig. S5a).

Staining:

Article Title: Lactose blocks intercellular spreading of Galectin-1 from cancer cells to T-cells and activates tumor immunological control
Article Snippet: .. Surprisingly, FACS staining with biotin-labeled recombinant Galectin-1 showed a more dramatic difference between control and B4GALT1 knockout OT-1 T-cells ( and Fig. S4c). .. RNA sequencing analysis revealed that galectin-1, galectin-3, and galectin-9 (Gal-1, Gal-3, Gal-9) were the most highly expressed galectins in OT-1 T-cells and MC38 cells (Fig. S5a).

Control:

Article Title: Lactose blocks intercellular spreading of Galectin-1 from cancer cells to T-cells and activates tumor immunological control
Article Snippet: .. Surprisingly, FACS staining with biotin-labeled recombinant Galectin-1 showed a more dramatic difference between control and B4GALT1 knockout OT-1 T-cells ( and Fig. S4c). .. RNA sequencing analysis revealed that galectin-1, galectin-3, and galectin-9 (Gal-1, Gal-3, Gal-9) were the most highly expressed galectins in OT-1 T-cells and MC38 cells (Fig. S5a).

Knock-Out:

Article Title: Lactose blocks intercellular spreading of Galectin-1 from cancer cells to T-cells and activates tumor immunological control
Article Snippet: .. Surprisingly, FACS staining with biotin-labeled recombinant Galectin-1 showed a more dramatic difference between control and B4GALT1 knockout OT-1 T-cells ( and Fig. S4c). .. RNA sequencing analysis revealed that galectin-1, galectin-3, and galectin-9 (Gal-1, Gal-3, Gal-9) were the most highly expressed galectins in OT-1 T-cells and MC38 cells (Fig. S5a).



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Image Search Results


Distributions of osteopontin and galectin-7 in controls (normal) vs. cases (benign and malignant). No statistically significant differences were observed between groups for osteopontin ( p = 0.562) or galectin-7 ( p = 0.138).

Journal: Journal of Clinical Medicine

Article Title: The Impact of Osteopontin and Galectin-7 on the Preoperative Diagnosis of Ovarian Tumors: A Case–Control Study

doi: 10.3390/jcm15062178

Figure Lengend Snippet: Distributions of osteopontin and galectin-7 in controls (normal) vs. cases (benign and malignant). No statistically significant differences were observed between groups for osteopontin ( p = 0.562) or galectin-7 ( p = 0.138).

Article Snippet: Osteopontin and galectin-7 concentrations were quantified from serum using commercially available ELISA kits by Boster Biological Technology (Pleasanton, CA, USA).

Techniques:

Galectin-7 levels in relation to age.

Journal: Journal of Clinical Medicine

Article Title: The Impact of Osteopontin and Galectin-7 on the Preoperative Diagnosis of Ovarian Tumors: A Case–Control Study

doi: 10.3390/jcm15062178

Figure Lengend Snippet: Galectin-7 levels in relation to age.

Article Snippet: Osteopontin and galectin-7 concentrations were quantified from serum using commercially available ELISA kits by Boster Biological Technology (Pleasanton, CA, USA).

Techniques:

Receiver operating characteristic (ROC) curves for osteopontin and galectin-7.

Journal: Journal of Clinical Medicine

Article Title: The Impact of Osteopontin and Galectin-7 on the Preoperative Diagnosis of Ovarian Tumors: A Case–Control Study

doi: 10.3390/jcm15062178

Figure Lengend Snippet: Receiver operating characteristic (ROC) curves for osteopontin and galectin-7.

Article Snippet: Osteopontin and galectin-7 concentrations were quantified from serum using commercially available ELISA kits by Boster Biological Technology (Pleasanton, CA, USA).

Techniques:

Receiver operating characteristic (ROC) curves for osteopontin (blue), galectin-7 (red), and the combined logistic regression model (green) in discriminating malignant from benign ovarian tumors.

Journal: Journal of Clinical Medicine

Article Title: The Impact of Osteopontin and Galectin-7 on the Preoperative Diagnosis of Ovarian Tumors: A Case–Control Study

doi: 10.3390/jcm15062178

Figure Lengend Snippet: Receiver operating characteristic (ROC) curves for osteopontin (blue), galectin-7 (red), and the combined logistic regression model (green) in discriminating malignant from benign ovarian tumors.

Article Snippet: Osteopontin and galectin-7 concentrations were quantified from serum using commercially available ELISA kits by Boster Biological Technology (Pleasanton, CA, USA).

Techniques:

Distributions of osteopontin and galectin-7 in controls (normal) vs. cases (benign and malignant). No statistically significant differences were observed between groups for osteopontin ( p = 0.562) or galectin-7 ( p = 0.138).

Journal: Journal of Clinical Medicine

Article Title: The Impact of Osteopontin and Galectin-7 on the Preoperative Diagnosis of Ovarian Tumors: A Case–Control Study

doi: 10.3390/jcm15062178

Figure Lengend Snippet: Distributions of osteopontin and galectin-7 in controls (normal) vs. cases (benign and malignant). No statistically significant differences were observed between groups for osteopontin ( p = 0.562) or galectin-7 ( p = 0.138).

Article Snippet: Osteopontin and galectin-7 concentrations were quantified from serum using commercially available ELISA kits by Boster Biological Technology (Pleasanton, CA, USA).

Techniques:

Receiver operating characteristic (ROC) curves for osteopontin and galectin-7.

Journal: Journal of Clinical Medicine

Article Title: The Impact of Osteopontin and Galectin-7 on the Preoperative Diagnosis of Ovarian Tumors: A Case–Control Study

doi: 10.3390/jcm15062178

Figure Lengend Snippet: Receiver operating characteristic (ROC) curves for osteopontin and galectin-7.

Article Snippet: Osteopontin and galectin-7 concentrations were quantified from serum using commercially available ELISA kits by Boster Biological Technology (Pleasanton, CA, USA).

Techniques:

Receiver operating characteristic (ROC) curves for osteopontin (blue), galectin-7 (red), and the combined logistic regression model (green) in discriminating malignant from benign ovarian tumors.

Journal: Journal of Clinical Medicine

Article Title: The Impact of Osteopontin and Galectin-7 on the Preoperative Diagnosis of Ovarian Tumors: A Case–Control Study

doi: 10.3390/jcm15062178

Figure Lengend Snippet: Receiver operating characteristic (ROC) curves for osteopontin (blue), galectin-7 (red), and the combined logistic regression model (green) in discriminating malignant from benign ovarian tumors.

Article Snippet: Osteopontin and galectin-7 concentrations were quantified from serum using commercially available ELISA kits by Boster Biological Technology (Pleasanton, CA, USA).

Techniques: